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STEMCELL Technologies Inc rosetteseptm human cd8 + t cell enrichment cocktail
Rosetteseptm Human Cd8 + T Cell Enrichment Cocktail, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rosetteseptm+human+cd8+++t+cell+enrichment+cocktail/rosettesep+human+b+cell+enrichment+cocktail/pmc12089405-180-12-20
Average 90 stars, based on 1 article reviews
rosetteseptm human cd8 + t cell enrichment cocktail - by Bioz Stars, 2026-09
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Isolation:

Article Title: Antibodies to TIGIT
Article Snippet: CD8+ T cells were isolated using the RosetteSepTM Human CD8+ T cell Enrichment Cocktail (Stemcell, Cat #15023) following manufacturer recommendation.

Article Title: Activation-induced thrombospondin-4 works with thrombospondin-1 to build cytotoxic supramolecular attack particles
Article Snippet: Cell culture and transfection of CTLs for confocal imaging Primary human CD8+ T lymphocytes were isolated from buffy coats of anonymous healthy donors and purified (>95% purity) by negative selection using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (STEMCELL Technologies).

Article Title: An AMBRA1, ULK1 and PP2A regulatory network regulates cytotoxic T cell differentiation via TFEB activation
Article Snippet: Human CD8 + T cells were purified from peripheral blood of healthy donors by negative selection using RosetteSepTM Human CD8 + T cells Enrichment Cocktail (STEMCELL Technologies, #15063) followed by density gradient centrifugation on Lympholyte (Cedarlane Laboratories, #CL5026).

Article Title: IFT20 regulates TFEB-dependent lytic granule biogenesis in cytotoxic T lymphocytes by orchestrating the MPR-dependent transport of granzyme B
Article Snippet: Primary CD8 + T cells were isolated by negative selection through the RosetteSepTM Human CD8 + T Cell Enrichment Cocktail (StemCell technologies, Vancouver, Canada) and centrifugation over a buoyant density medium (Lympholyte Cell Separation Medium, Euroclone, Milan, Italy).

Article Title: Siglec-9 ECD fusion molecules
Article Snippet: Autologous CD8+ T cells were isolated from blood using a RosetteSepTM Human CD8+ T Cell Enrichment Cocktail kit (StemCell).

Article Title: Monoclonal antibodies directed against programmed death-1 protein and their use in medicine
Article Snippet: Human CD8+ T cells were purified (enriched) from buffy coats obtained from healthy volunteers (Sanquin) by negative selection using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (Stemcell Technologies, cat. no. 15023C.2) or by positive selection via magnetic activated cell sorting (MACS), using CD8 MicroBeads (Miltenyi Biotec, cat. no. 130-045-201) and LS columns (Miltenyi Biotec, cat. no. 130-042-401), all according to the manufacturer's instructions.

Article Title: Disrupting EDEM3‐induced M2‐like macrophage trafficking by glucose restriction overcomes resistance to PD‐1/PD‐L1 blockade
Article Snippet: T cells were isolated using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (STEMCELL, Cat#:15023C) following the manufacturer’s instructions.

Article Title: The surfaceome of multiple myeloma cells suggests potential immunotherapeutic strategies and protein markers of drug resistance
Article Snippet: Primary human T cells were purified from the leukapheresis products of anonymous healthy blood donors using RosetteSepTM Human CD8+ T Cell Enrichment Cocktail and EasySep kit (STEMCELL Technologies, Inc.) under an institutional review board–exempt protocol in accordance with the U.S. Common Rule (Category 4) and were resuspended in T cell media (CTSTM OpTmizerTM T Cell Expansion SFM, GibcoTM) supplemented with 5% of filtered, heat inactivated and sterile human AB serum, 1% Pen-strep, and 1%, glutamax 1% (GibcoTM), IL-7 and IL-15 were added to media at 10 ng/mL concentration (PeproTech).

Selection:

Article Title: Antibodies to TIGIT
Article Snippet: CD8+ T cells were isolated using the RosetteSepTM Human CD8+ T cell Enrichment Cocktail (Stemcell, Cat #15023) following manufacturer recommendation.

Article Title: Activation-induced thrombospondin-4 works with thrombospondin-1 to build cytotoxic supramolecular attack particles
Article Snippet: Cell culture and transfection of CTLs for confocal imaging Primary human CD8+ T lymphocytes were isolated from buffy coats of anonymous healthy donors and purified (>95% purity) by negative selection using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (STEMCELL Technologies).

Article Title: An AMBRA1, ULK1 and PP2A regulatory network regulates cytotoxic T cell differentiation via TFEB activation
Article Snippet: Human CD8 + T cells were purified from peripheral blood of healthy donors by negative selection using RosetteSepTM Human CD8 + T cells Enrichment Cocktail (STEMCELL Technologies, #15063) followed by density gradient centrifugation on Lympholyte (Cedarlane Laboratories, #CL5026).

Article Title: IFT20 regulates TFEB-dependent lytic granule biogenesis in cytotoxic T lymphocytes by orchestrating the MPR-dependent transport of granzyme B
Article Snippet: Primary CD8 + T cells were isolated by negative selection through the RosetteSepTM Human CD8 + T Cell Enrichment Cocktail (StemCell technologies, Vancouver, Canada) and centrifugation over a buoyant density medium (Lympholyte Cell Separation Medium, Euroclone, Milan, Italy).

Article Title: Siglec-9 ECD fusion molecules
Article Snippet: Autologous CD8+ T cells were isolated from blood using a RosetteSepTM Human CD8+ T Cell Enrichment Cocktail kit (StemCell).

Article Title: Monoclonal antibodies directed against programmed death-1 protein and their use in medicine
Article Snippet: Human CD8+ T cells were purified (enriched) from buffy coats obtained from healthy volunteers (Sanquin) by negative selection using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (Stemcell Technologies, cat. no. 15023C.2) or by positive selection via magnetic activated cell sorting (MACS), using CD8 MicroBeads (Miltenyi Biotec, cat. no. 130-045-201) and LS columns (Miltenyi Biotec, cat. no. 130-042-401), all according to the manufacturer's instructions.

Article Title: Disrupting EDEM3‐induced M2‐like macrophage trafficking by glucose restriction overcomes resistance to PD‐1/PD‐L1 blockade
Article Snippet: T cells were isolated using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (STEMCELL, Cat#:15023C) following the manufacturer’s instructions.

Article Title: The surfaceome of multiple myeloma cells suggests potential immunotherapeutic strategies and protein markers of drug resistance
Article Snippet: Primary human T cells were purified from the leukapheresis products of anonymous healthy blood donors using RosetteSepTM Human CD8+ T Cell Enrichment Cocktail and EasySep kit (STEMCELL Technologies, Inc.) under an institutional review board–exempt protocol in accordance with the U.S. Common Rule (Category 4) and were resuspended in T cell media (CTSTM OpTmizerTM T Cell Expansion SFM, GibcoTM) supplemented with 5% of filtered, heat inactivated and sterile human AB serum, 1% Pen-strep, and 1%, glutamax 1% (GibcoTM), IL-7 and IL-15 were added to media at 10 ng/mL concentration (PeproTech).

Centrifugation:

Article Title: Antibodies to TIGIT
Article Snippet: CD8+ T cells were isolated using the RosetteSepTM Human CD8+ T cell Enrichment Cocktail (Stemcell, Cat #15023) following manufacturer recommendation.

Article Title: Activation-induced thrombospondin-4 works with thrombospondin-1 to build cytotoxic supramolecular attack particles
Article Snippet: Cell culture and transfection of CTLs for confocal imaging Primary human CD8+ T lymphocytes were isolated from buffy coats of anonymous healthy donors and purified (>95% purity) by negative selection using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (STEMCELL Technologies).

Article Title: An AMBRA1, ULK1 and PP2A regulatory network regulates cytotoxic T cell differentiation via TFEB activation
Article Snippet: Human CD8 + T cells were purified from peripheral blood of healthy donors by negative selection using RosetteSepTM Human CD8 + T cells Enrichment Cocktail (STEMCELL Technologies, #15063) followed by density gradient centrifugation on Lympholyte (Cedarlane Laboratories, #CL5026).

Article Title: IFT20 regulates TFEB-dependent lytic granule biogenesis in cytotoxic T lymphocytes by orchestrating the MPR-dependent transport of granzyme B
Article Snippet: Primary CD8 + T cells were isolated by negative selection through the RosetteSepTM Human CD8 + T Cell Enrichment Cocktail (StemCell technologies, Vancouver, Canada) and centrifugation over a buoyant density medium (Lympholyte Cell Separation Medium, Euroclone, Milan, Italy).

Article Title: Siglec-9 ECD fusion molecules
Article Snippet: Autologous CD8+ T cells were isolated from blood using a RosetteSepTM Human CD8+ T Cell Enrichment Cocktail kit (StemCell).

Article Title: Monoclonal antibodies directed against programmed death-1 protein and their use in medicine
Article Snippet: Human CD8+ T cells were purified (enriched) from buffy coats obtained from healthy volunteers (Sanquin) by negative selection using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (Stemcell Technologies, cat. no. 15023C.2) or by positive selection via magnetic activated cell sorting (MACS), using CD8 MicroBeads (Miltenyi Biotec, cat. no. 130-045-201) and LS columns (Miltenyi Biotec, cat. no. 130-042-401), all according to the manufacturer's instructions.

Article Title: Disrupting EDEM3‐induced M2‐like macrophage trafficking by glucose restriction overcomes resistance to PD‐1/PD‐L1 blockade
Article Snippet: T cells were isolated using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (STEMCELL, Cat#:15023C) following the manufacturer’s instructions.

Article Title: The surfaceome of multiple myeloma cells suggests potential immunotherapeutic strategies and protein markers of drug resistance
Article Snippet: Primary human T cells were purified from the leukapheresis products of anonymous healthy blood donors using RosetteSepTM Human CD8+ T Cell Enrichment Cocktail and EasySep kit (STEMCELL Technologies, Inc.) under an institutional review board–exempt protocol in accordance with the U.S. Common Rule (Category 4) and were resuspended in T cell media (CTSTM OpTmizerTM T Cell Expansion SFM, GibcoTM) supplemented with 5% of filtered, heat inactivated and sterile human AB serum, 1% Pen-strep, and 1%, glutamax 1% (GibcoTM), IL-7 and IL-15 were added to media at 10 ng/mL concentration (PeproTech).

Cell Culture:

Article Title: Antibodies to TIGIT
Article Snippet: CD8+ T cells were isolated using the RosetteSepTM Human CD8+ T cell Enrichment Cocktail (Stemcell, Cat #15023) following manufacturer recommendation.

Article Title: Activation-induced thrombospondin-4 works with thrombospondin-1 to build cytotoxic supramolecular attack particles
Article Snippet: Cell culture and transfection of CTLs for confocal imaging Primary human CD8+ T lymphocytes were isolated from buffy coats of anonymous healthy donors and purified (>95% purity) by negative selection using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (STEMCELL Technologies).

Article Title: An AMBRA1, ULK1 and PP2A regulatory network regulates cytotoxic T cell differentiation via TFEB activation
Article Snippet: Human CD8 + T cells were purified from peripheral blood of healthy donors by negative selection using RosetteSepTM Human CD8 + T cells Enrichment Cocktail (STEMCELL Technologies, #15063) followed by density gradient centrifugation on Lympholyte (Cedarlane Laboratories, #CL5026).

Article Title: IFT20 regulates TFEB-dependent lytic granule biogenesis in cytotoxic T lymphocytes by orchestrating the MPR-dependent transport of granzyme B
Article Snippet: Primary CD8 + T cells were isolated by negative selection through the RosetteSepTM Human CD8 + T Cell Enrichment Cocktail (StemCell technologies, Vancouver, Canada) and centrifugation over a buoyant density medium (Lympholyte Cell Separation Medium, Euroclone, Milan, Italy).

Article Title: Siglec-9 ECD fusion molecules
Article Snippet: Autologous CD8+ T cells were isolated from blood using a RosetteSepTM Human CD8+ T Cell Enrichment Cocktail kit (StemCell).

Article Title: Monoclonal antibodies directed against programmed death-1 protein and their use in medicine
Article Snippet: Human CD8+ T cells were purified (enriched) from buffy coats obtained from healthy volunteers (Sanquin) by negative selection using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (Stemcell Technologies, cat. no. 15023C.2) or by positive selection via magnetic activated cell sorting (MACS), using CD8 MicroBeads (Miltenyi Biotec, cat. no. 130-045-201) and LS columns (Miltenyi Biotec, cat. no. 130-042-401), all according to the manufacturer's instructions.

Article Title: Disrupting EDEM3‐induced M2‐like macrophage trafficking by glucose restriction overcomes resistance to PD‐1/PD‐L1 blockade
Article Snippet: T cells were isolated using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (STEMCELL, Cat#:15023C) following the manufacturer’s instructions.

Article Title: The surfaceome of multiple myeloma cells suggests potential immunotherapeutic strategies and protein markers of drug resistance
Article Snippet: Primary human T cells were purified from the leukapheresis products of anonymous healthy blood donors using RosetteSepTM Human CD8+ T Cell Enrichment Cocktail and EasySep kit (STEMCELL Technologies, Inc.) under an institutional review board–exempt protocol in accordance with the U.S. Common Rule (Category 4) and were resuspended in T cell media (CTSTM OpTmizerTM T Cell Expansion SFM, GibcoTM) supplemented with 5% of filtered, heat inactivated and sterile human AB serum, 1% Pen-strep, and 1%, glutamax 1% (GibcoTM), IL-7 and IL-15 were added to media at 10 ng/mL concentration (PeproTech).

Transfection:

Article Title: Antibodies to TIGIT
Article Snippet: CD8+ T cells were isolated using the RosetteSepTM Human CD8+ T cell Enrichment Cocktail (Stemcell, Cat #15023) following manufacturer recommendation.

Article Title: Activation-induced thrombospondin-4 works with thrombospondin-1 to build cytotoxic supramolecular attack particles
Article Snippet: Cell culture and transfection of CTLs for confocal imaging Primary human CD8+ T lymphocytes were isolated from buffy coats of anonymous healthy donors and purified (>95% purity) by negative selection using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (STEMCELL Technologies).

Article Title: An AMBRA1, ULK1 and PP2A regulatory network regulates cytotoxic T cell differentiation via TFEB activation
Article Snippet: Human CD8 + T cells were purified from peripheral blood of healthy donors by negative selection using RosetteSepTM Human CD8 + T cells Enrichment Cocktail (STEMCELL Technologies, #15063) followed by density gradient centrifugation on Lympholyte (Cedarlane Laboratories, #CL5026).

Article Title: IFT20 regulates TFEB-dependent lytic granule biogenesis in cytotoxic T lymphocytes by orchestrating the MPR-dependent transport of granzyme B
Article Snippet: Primary CD8 + T cells were isolated by negative selection through the RosetteSepTM Human CD8 + T Cell Enrichment Cocktail (StemCell technologies, Vancouver, Canada) and centrifugation over a buoyant density medium (Lympholyte Cell Separation Medium, Euroclone, Milan, Italy).

Article Title: Siglec-9 ECD fusion molecules
Article Snippet: Autologous CD8+ T cells were isolated from blood using a RosetteSepTM Human CD8+ T Cell Enrichment Cocktail kit (StemCell).

Article Title: Monoclonal antibodies directed against programmed death-1 protein and their use in medicine
Article Snippet: Human CD8+ T cells were purified (enriched) from buffy coats obtained from healthy volunteers (Sanquin) by negative selection using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (Stemcell Technologies, cat. no. 15023C.2) or by positive selection via magnetic activated cell sorting (MACS), using CD8 MicroBeads (Miltenyi Biotec, cat. no. 130-045-201) and LS columns (Miltenyi Biotec, cat. no. 130-042-401), all according to the manufacturer's instructions.

Article Title: Disrupting EDEM3‐induced M2‐like macrophage trafficking by glucose restriction overcomes resistance to PD‐1/PD‐L1 blockade
Article Snippet: T cells were isolated using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (STEMCELL, Cat#:15023C) following the manufacturer’s instructions.

Article Title: The surfaceome of multiple myeloma cells suggests potential immunotherapeutic strategies and protein markers of drug resistance
Article Snippet: Primary human T cells were purified from the leukapheresis products of anonymous healthy blood donors using RosetteSepTM Human CD8+ T Cell Enrichment Cocktail and EasySep kit (STEMCELL Technologies, Inc.) under an institutional review board–exempt protocol in accordance with the U.S. Common Rule (Category 4) and were resuspended in T cell media (CTSTM OpTmizerTM T Cell Expansion SFM, GibcoTM) supplemented with 5% of filtered, heat inactivated and sterile human AB serum, 1% Pen-strep, and 1%, glutamax 1% (GibcoTM), IL-7 and IL-15 were added to media at 10 ng/mL concentration (PeproTech).

Imaging:

Article Title: Antibodies to TIGIT
Article Snippet: CD8+ T cells were isolated using the RosetteSepTM Human CD8+ T cell Enrichment Cocktail (Stemcell, Cat #15023) following manufacturer recommendation.

Article Title: Activation-induced thrombospondin-4 works with thrombospondin-1 to build cytotoxic supramolecular attack particles
Article Snippet: Cell culture and transfection of CTLs for confocal imaging Primary human CD8+ T lymphocytes were isolated from buffy coats of anonymous healthy donors and purified (>95% purity) by negative selection using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (STEMCELL Technologies).

Article Title: An AMBRA1, ULK1 and PP2A regulatory network regulates cytotoxic T cell differentiation via TFEB activation
Article Snippet: Human CD8 + T cells were purified from peripheral blood of healthy donors by negative selection using RosetteSepTM Human CD8 + T cells Enrichment Cocktail (STEMCELL Technologies, #15063) followed by density gradient centrifugation on Lympholyte (Cedarlane Laboratories, #CL5026).

Article Title: IFT20 regulates TFEB-dependent lytic granule biogenesis in cytotoxic T lymphocytes by orchestrating the MPR-dependent transport of granzyme B
Article Snippet: Primary CD8 + T cells were isolated by negative selection through the RosetteSepTM Human CD8 + T Cell Enrichment Cocktail (StemCell technologies, Vancouver, Canada) and centrifugation over a buoyant density medium (Lympholyte Cell Separation Medium, Euroclone, Milan, Italy).

Article Title: Siglec-9 ECD fusion molecules
Article Snippet: Autologous CD8+ T cells were isolated from blood using a RosetteSepTM Human CD8+ T Cell Enrichment Cocktail kit (StemCell).

Article Title: Monoclonal antibodies directed against programmed death-1 protein and their use in medicine
Article Snippet: Human CD8+ T cells were purified (enriched) from buffy coats obtained from healthy volunteers (Sanquin) by negative selection using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (Stemcell Technologies, cat. no. 15023C.2) or by positive selection via magnetic activated cell sorting (MACS), using CD8 MicroBeads (Miltenyi Biotec, cat. no. 130-045-201) and LS columns (Miltenyi Biotec, cat. no. 130-042-401), all according to the manufacturer's instructions.

Article Title: Disrupting EDEM3‐induced M2‐like macrophage trafficking by glucose restriction overcomes resistance to PD‐1/PD‐L1 blockade
Article Snippet: T cells were isolated using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (STEMCELL, Cat#:15023C) following the manufacturer’s instructions.

Article Title: The surfaceome of multiple myeloma cells suggests potential immunotherapeutic strategies and protein markers of drug resistance
Article Snippet: Primary human T cells were purified from the leukapheresis products of anonymous healthy blood donors using RosetteSepTM Human CD8+ T Cell Enrichment Cocktail and EasySep kit (STEMCELL Technologies, Inc.) under an institutional review board–exempt protocol in accordance with the U.S. Common Rule (Category 4) and were resuspended in T cell media (CTSTM OpTmizerTM T Cell Expansion SFM, GibcoTM) supplemented with 5% of filtered, heat inactivated and sterile human AB serum, 1% Pen-strep, and 1%, glutamax 1% (GibcoTM), IL-7 and IL-15 were added to media at 10 ng/mL concentration (PeproTech).

Purification:

Article Title: Antibodies to TIGIT
Article Snippet: CD8+ T cells were isolated using the RosetteSepTM Human CD8+ T cell Enrichment Cocktail (Stemcell, Cat #15023) following manufacturer recommendation.

Article Title: Activation-induced thrombospondin-4 works with thrombospondin-1 to build cytotoxic supramolecular attack particles
Article Snippet: Cell culture and transfection of CTLs for confocal imaging Primary human CD8+ T lymphocytes were isolated from buffy coats of anonymous healthy donors and purified (>95% purity) by negative selection using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (STEMCELL Technologies).

Article Title: An AMBRA1, ULK1 and PP2A regulatory network regulates cytotoxic T cell differentiation via TFEB activation
Article Snippet: Human CD8 + T cells were purified from peripheral blood of healthy donors by negative selection using RosetteSepTM Human CD8 + T cells Enrichment Cocktail (STEMCELL Technologies, #15063) followed by density gradient centrifugation on Lympholyte (Cedarlane Laboratories, #CL5026).

Article Title: IFT20 regulates TFEB-dependent lytic granule biogenesis in cytotoxic T lymphocytes by orchestrating the MPR-dependent transport of granzyme B
Article Snippet: Primary CD8 + T cells were isolated by negative selection through the RosetteSepTM Human CD8 + T Cell Enrichment Cocktail (StemCell technologies, Vancouver, Canada) and centrifugation over a buoyant density medium (Lympholyte Cell Separation Medium, Euroclone, Milan, Italy).

Article Title: Siglec-9 ECD fusion molecules
Article Snippet: Autologous CD8+ T cells were isolated from blood using a RosetteSepTM Human CD8+ T Cell Enrichment Cocktail kit (StemCell).

Article Title: Monoclonal antibodies directed against programmed death-1 protein and their use in medicine
Article Snippet: Human CD8+ T cells were purified (enriched) from buffy coats obtained from healthy volunteers (Sanquin) by negative selection using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (Stemcell Technologies, cat. no. 15023C.2) or by positive selection via magnetic activated cell sorting (MACS), using CD8 MicroBeads (Miltenyi Biotec, cat. no. 130-045-201) and LS columns (Miltenyi Biotec, cat. no. 130-042-401), all according to the manufacturer's instructions.

Article Title: Disrupting EDEM3‐induced M2‐like macrophage trafficking by glucose restriction overcomes resistance to PD‐1/PD‐L1 blockade
Article Snippet: T cells were isolated using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (STEMCELL, Cat#:15023C) following the manufacturer’s instructions.

Article Title: The surfaceome of multiple myeloma cells suggests potential immunotherapeutic strategies and protein markers of drug resistance
Article Snippet: Primary human T cells were purified from the leukapheresis products of anonymous healthy blood donors using RosetteSepTM Human CD8+ T Cell Enrichment Cocktail and EasySep kit (STEMCELL Technologies, Inc.) under an institutional review board–exempt protocol in accordance with the U.S. Common Rule (Category 4) and were resuspended in T cell media (CTSTM OpTmizerTM T Cell Expansion SFM, GibcoTM) supplemented with 5% of filtered, heat inactivated and sterile human AB serum, 1% Pen-strep, and 1%, glutamax 1% (GibcoTM), IL-7 and IL-15 were added to media at 10 ng/mL concentration (PeproTech).

Gradient Centrifugation:

Article Title: Antibodies to TIGIT
Article Snippet: CD8+ T cells were isolated using the RosetteSepTM Human CD8+ T cell Enrichment Cocktail (Stemcell, Cat #15023) following manufacturer recommendation.

Article Title: Activation-induced thrombospondin-4 works with thrombospondin-1 to build cytotoxic supramolecular attack particles
Article Snippet: Cell culture and transfection of CTLs for confocal imaging Primary human CD8+ T lymphocytes were isolated from buffy coats of anonymous healthy donors and purified (>95% purity) by negative selection using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (STEMCELL Technologies).

Article Title: An AMBRA1, ULK1 and PP2A regulatory network regulates cytotoxic T cell differentiation via TFEB activation
Article Snippet: Human CD8 + T cells were purified from peripheral blood of healthy donors by negative selection using RosetteSepTM Human CD8 + T cells Enrichment Cocktail (STEMCELL Technologies, #15063) followed by density gradient centrifugation on Lympholyte (Cedarlane Laboratories, #CL5026).

Article Title: IFT20 regulates TFEB-dependent lytic granule biogenesis in cytotoxic T lymphocytes by orchestrating the MPR-dependent transport of granzyme B
Article Snippet: Primary CD8 + T cells were isolated by negative selection through the RosetteSepTM Human CD8 + T Cell Enrichment Cocktail (StemCell technologies, Vancouver, Canada) and centrifugation over a buoyant density medium (Lympholyte Cell Separation Medium, Euroclone, Milan, Italy).

Article Title: Siglec-9 ECD fusion molecules
Article Snippet: Autologous CD8+ T cells were isolated from blood using a RosetteSepTM Human CD8+ T Cell Enrichment Cocktail kit (StemCell).

Article Title: Monoclonal antibodies directed against programmed death-1 protein and their use in medicine
Article Snippet: Human CD8+ T cells were purified (enriched) from buffy coats obtained from healthy volunteers (Sanquin) by negative selection using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (Stemcell Technologies, cat. no. 15023C.2) or by positive selection via magnetic activated cell sorting (MACS), using CD8 MicroBeads (Miltenyi Biotec, cat. no. 130-045-201) and LS columns (Miltenyi Biotec, cat. no. 130-042-401), all according to the manufacturer's instructions.

Article Title: Disrupting EDEM3‐induced M2‐like macrophage trafficking by glucose restriction overcomes resistance to PD‐1/PD‐L1 blockade
Article Snippet: T cells were isolated using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (STEMCELL, Cat#:15023C) following the manufacturer’s instructions.

Article Title: The surfaceome of multiple myeloma cells suggests potential immunotherapeutic strategies and protein markers of drug resistance
Article Snippet: Primary human T cells were purified from the leukapheresis products of anonymous healthy blood donors using RosetteSepTM Human CD8+ T Cell Enrichment Cocktail and EasySep kit (STEMCELL Technologies, Inc.) under an institutional review board–exempt protocol in accordance with the U.S. Common Rule (Category 4) and were resuspended in T cell media (CTSTM OpTmizerTM T Cell Expansion SFM, GibcoTM) supplemented with 5% of filtered, heat inactivated and sterile human AB serum, 1% Pen-strep, and 1%, glutamax 1% (GibcoTM), IL-7 and IL-15 were added to media at 10 ng/mL concentration (PeproTech).

FACS:

Article Title: Antibodies to TIGIT
Article Snippet: CD8+ T cells were isolated using the RosetteSepTM Human CD8+ T cell Enrichment Cocktail (Stemcell, Cat #15023) following manufacturer recommendation.

Article Title: Activation-induced thrombospondin-4 works with thrombospondin-1 to build cytotoxic supramolecular attack particles
Article Snippet: Cell culture and transfection of CTLs for confocal imaging Primary human CD8+ T lymphocytes were isolated from buffy coats of anonymous healthy donors and purified (>95% purity) by negative selection using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (STEMCELL Technologies).

Article Title: An AMBRA1, ULK1 and PP2A regulatory network regulates cytotoxic T cell differentiation via TFEB activation
Article Snippet: Human CD8 + T cells were purified from peripheral blood of healthy donors by negative selection using RosetteSepTM Human CD8 + T cells Enrichment Cocktail (STEMCELL Technologies, #15063) followed by density gradient centrifugation on Lympholyte (Cedarlane Laboratories, #CL5026).

Article Title: IFT20 regulates TFEB-dependent lytic granule biogenesis in cytotoxic T lymphocytes by orchestrating the MPR-dependent transport of granzyme B
Article Snippet: Primary CD8 + T cells were isolated by negative selection through the RosetteSepTM Human CD8 + T Cell Enrichment Cocktail (StemCell technologies, Vancouver, Canada) and centrifugation over a buoyant density medium (Lympholyte Cell Separation Medium, Euroclone, Milan, Italy).

Article Title: Siglec-9 ECD fusion molecules
Article Snippet: Autologous CD8+ T cells were isolated from blood using a RosetteSepTM Human CD8+ T Cell Enrichment Cocktail kit (StemCell).

Article Title: Monoclonal antibodies directed against programmed death-1 protein and their use in medicine
Article Snippet: Human CD8+ T cells were purified (enriched) from buffy coats obtained from healthy volunteers (Sanquin) by negative selection using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (Stemcell Technologies, cat. no. 15023C.2) or by positive selection via magnetic activated cell sorting (MACS), using CD8 MicroBeads (Miltenyi Biotec, cat. no. 130-045-201) and LS columns (Miltenyi Biotec, cat. no. 130-042-401), all according to the manufacturer's instructions.

Article Title: Disrupting EDEM3‐induced M2‐like macrophage trafficking by glucose restriction overcomes resistance to PD‐1/PD‐L1 blockade
Article Snippet: T cells were isolated using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (STEMCELL, Cat#:15023C) following the manufacturer’s instructions.

Article Title: The surfaceome of multiple myeloma cells suggests potential immunotherapeutic strategies and protein markers of drug resistance
Article Snippet: Primary human T cells were purified from the leukapheresis products of anonymous healthy blood donors using RosetteSepTM Human CD8+ T Cell Enrichment Cocktail and EasySep kit (STEMCELL Technologies, Inc.) under an institutional review board–exempt protocol in accordance with the U.S. Common Rule (Category 4) and were resuspended in T cell media (CTSTM OpTmizerTM T Cell Expansion SFM, GibcoTM) supplemented with 5% of filtered, heat inactivated and sterile human AB serum, 1% Pen-strep, and 1%, glutamax 1% (GibcoTM), IL-7 and IL-15 were added to media at 10 ng/mL concentration (PeproTech).

Magnetic Cell Separation:

Article Title: Antibodies to TIGIT
Article Snippet: CD8+ T cells were isolated using the RosetteSepTM Human CD8+ T cell Enrichment Cocktail (Stemcell, Cat #15023) following manufacturer recommendation.

Article Title: Activation-induced thrombospondin-4 works with thrombospondin-1 to build cytotoxic supramolecular attack particles
Article Snippet: Cell culture and transfection of CTLs for confocal imaging Primary human CD8+ T lymphocytes were isolated from buffy coats of anonymous healthy donors and purified (>95% purity) by negative selection using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (STEMCELL Technologies).

Article Title: An AMBRA1, ULK1 and PP2A regulatory network regulates cytotoxic T cell differentiation via TFEB activation
Article Snippet: Human CD8 + T cells were purified from peripheral blood of healthy donors by negative selection using RosetteSepTM Human CD8 + T cells Enrichment Cocktail (STEMCELL Technologies, #15063) followed by density gradient centrifugation on Lympholyte (Cedarlane Laboratories, #CL5026).

Article Title: IFT20 regulates TFEB-dependent lytic granule biogenesis in cytotoxic T lymphocytes by orchestrating the MPR-dependent transport of granzyme B
Article Snippet: Primary CD8 + T cells were isolated by negative selection through the RosetteSepTM Human CD8 + T Cell Enrichment Cocktail (StemCell technologies, Vancouver, Canada) and centrifugation over a buoyant density medium (Lympholyte Cell Separation Medium, Euroclone, Milan, Italy).

Article Title: Siglec-9 ECD fusion molecules
Article Snippet: Autologous CD8+ T cells were isolated from blood using a RosetteSepTM Human CD8+ T Cell Enrichment Cocktail kit (StemCell).

Article Title: Monoclonal antibodies directed against programmed death-1 protein and their use in medicine
Article Snippet: Human CD8+ T cells were purified (enriched) from buffy coats obtained from healthy volunteers (Sanquin) by negative selection using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (Stemcell Technologies, cat. no. 15023C.2) or by positive selection via magnetic activated cell sorting (MACS), using CD8 MicroBeads (Miltenyi Biotec, cat. no. 130-045-201) and LS columns (Miltenyi Biotec, cat. no. 130-042-401), all according to the manufacturer's instructions.

Article Title: Disrupting EDEM3‐induced M2‐like macrophage trafficking by glucose restriction overcomes resistance to PD‐1/PD‐L1 blockade
Article Snippet: T cells were isolated using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (STEMCELL, Cat#:15023C) following the manufacturer’s instructions.

Article Title: The surfaceome of multiple myeloma cells suggests potential immunotherapeutic strategies and protein markers of drug resistance
Article Snippet: Primary human T cells were purified from the leukapheresis products of anonymous healthy blood donors using RosetteSepTM Human CD8+ T Cell Enrichment Cocktail and EasySep kit (STEMCELL Technologies, Inc.) under an institutional review board–exempt protocol in accordance with the U.S. Common Rule (Category 4) and were resuspended in T cell media (CTSTM OpTmizerTM T Cell Expansion SFM, GibcoTM) supplemented with 5% of filtered, heat inactivated and sterile human AB serum, 1% Pen-strep, and 1%, glutamax 1% (GibcoTM), IL-7 and IL-15 were added to media at 10 ng/mL concentration (PeproTech).

Sterility:

Article Title: Antibodies to TIGIT
Article Snippet: CD8+ T cells were isolated using the RosetteSepTM Human CD8+ T cell Enrichment Cocktail (Stemcell, Cat #15023) following manufacturer recommendation.

Article Title: Activation-induced thrombospondin-4 works with thrombospondin-1 to build cytotoxic supramolecular attack particles
Article Snippet: Cell culture and transfection of CTLs for confocal imaging Primary human CD8+ T lymphocytes were isolated from buffy coats of anonymous healthy donors and purified (>95% purity) by negative selection using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (STEMCELL Technologies).

Article Title: An AMBRA1, ULK1 and PP2A regulatory network regulates cytotoxic T cell differentiation via TFEB activation
Article Snippet: Human CD8 + T cells were purified from peripheral blood of healthy donors by negative selection using RosetteSepTM Human CD8 + T cells Enrichment Cocktail (STEMCELL Technologies, #15063) followed by density gradient centrifugation on Lympholyte (Cedarlane Laboratories, #CL5026).

Article Title: IFT20 regulates TFEB-dependent lytic granule biogenesis in cytotoxic T lymphocytes by orchestrating the MPR-dependent transport of granzyme B
Article Snippet: Primary CD8 + T cells were isolated by negative selection through the RosetteSepTM Human CD8 + T Cell Enrichment Cocktail (StemCell technologies, Vancouver, Canada) and centrifugation over a buoyant density medium (Lympholyte Cell Separation Medium, Euroclone, Milan, Italy).

Article Title: Siglec-9 ECD fusion molecules
Article Snippet: Autologous CD8+ T cells were isolated from blood using a RosetteSepTM Human CD8+ T Cell Enrichment Cocktail kit (StemCell).

Article Title: Monoclonal antibodies directed against programmed death-1 protein and their use in medicine
Article Snippet: Human CD8+ T cells were purified (enriched) from buffy coats obtained from healthy volunteers (Sanquin) by negative selection using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (Stemcell Technologies, cat. no. 15023C.2) or by positive selection via magnetic activated cell sorting (MACS), using CD8 MicroBeads (Miltenyi Biotec, cat. no. 130-045-201) and LS columns (Miltenyi Biotec, cat. no. 130-042-401), all according to the manufacturer's instructions.

Article Title: Disrupting EDEM3‐induced M2‐like macrophage trafficking by glucose restriction overcomes resistance to PD‐1/PD‐L1 blockade
Article Snippet: T cells were isolated using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (STEMCELL, Cat#:15023C) following the manufacturer’s instructions.

Article Title: The surfaceome of multiple myeloma cells suggests potential immunotherapeutic strategies and protein markers of drug resistance
Article Snippet: Primary human T cells were purified from the leukapheresis products of anonymous healthy blood donors using RosetteSepTM Human CD8+ T Cell Enrichment Cocktail and EasySep kit (STEMCELL Technologies, Inc.) under an institutional review board–exempt protocol in accordance with the U.S. Common Rule (Category 4) and were resuspended in T cell media (CTSTM OpTmizerTM T Cell Expansion SFM, GibcoTM) supplemented with 5% of filtered, heat inactivated and sterile human AB serum, 1% Pen-strep, and 1%, glutamax 1% (GibcoTM), IL-7 and IL-15 were added to media at 10 ng/mL concentration (PeproTech).

Concentration Assay:

Article Title: Antibodies to TIGIT
Article Snippet: CD8+ T cells were isolated using the RosetteSepTM Human CD8+ T cell Enrichment Cocktail (Stemcell, Cat #15023) following manufacturer recommendation.

Article Title: Activation-induced thrombospondin-4 works with thrombospondin-1 to build cytotoxic supramolecular attack particles
Article Snippet: Cell culture and transfection of CTLs for confocal imaging Primary human CD8+ T lymphocytes were isolated from buffy coats of anonymous healthy donors and purified (>95% purity) by negative selection using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (STEMCELL Technologies).

Article Title: An AMBRA1, ULK1 and PP2A regulatory network regulates cytotoxic T cell differentiation via TFEB activation
Article Snippet: Human CD8 + T cells were purified from peripheral blood of healthy donors by negative selection using RosetteSepTM Human CD8 + T cells Enrichment Cocktail (STEMCELL Technologies, #15063) followed by density gradient centrifugation on Lympholyte (Cedarlane Laboratories, #CL5026).

Article Title: IFT20 regulates TFEB-dependent lytic granule biogenesis in cytotoxic T lymphocytes by orchestrating the MPR-dependent transport of granzyme B
Article Snippet: Primary CD8 + T cells were isolated by negative selection through the RosetteSepTM Human CD8 + T Cell Enrichment Cocktail (StemCell technologies, Vancouver, Canada) and centrifugation over a buoyant density medium (Lympholyte Cell Separation Medium, Euroclone, Milan, Italy).

Article Title: Siglec-9 ECD fusion molecules
Article Snippet: Autologous CD8+ T cells were isolated from blood using a RosetteSepTM Human CD8+ T Cell Enrichment Cocktail kit (StemCell).

Article Title: Monoclonal antibodies directed against programmed death-1 protein and their use in medicine
Article Snippet: Human CD8+ T cells were purified (enriched) from buffy coats obtained from healthy volunteers (Sanquin) by negative selection using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (Stemcell Technologies, cat. no. 15023C.2) or by positive selection via magnetic activated cell sorting (MACS), using CD8 MicroBeads (Miltenyi Biotec, cat. no. 130-045-201) and LS columns (Miltenyi Biotec, cat. no. 130-042-401), all according to the manufacturer's instructions.

Article Title: Disrupting EDEM3‐induced M2‐like macrophage trafficking by glucose restriction overcomes resistance to PD‐1/PD‐L1 blockade
Article Snippet: T cells were isolated using the RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (STEMCELL, Cat#:15023C) following the manufacturer’s instructions.

Article Title: The surfaceome of multiple myeloma cells suggests potential immunotherapeutic strategies and protein markers of drug resistance
Article Snippet: Primary human T cells were purified from the leukapheresis products of anonymous healthy blood donors using RosetteSepTM Human CD8+ T Cell Enrichment Cocktail and EasySep kit (STEMCELL Technologies, Inc.) under an institutional review board–exempt protocol in accordance with the U.S. Common Rule (Category 4) and were resuspended in T cell media (CTSTM OpTmizerTM T Cell Expansion SFM, GibcoTM) supplemented with 5% of filtered, heat inactivated and sterile human AB serum, 1% Pen-strep, and 1%, glutamax 1% (GibcoTM), IL-7 and IL-15 were added to media at 10 ng/mL concentration (PeproTech).



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